FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l08n02_143.35200000073ce4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l08n02_143.35200000073ce4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30114336
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG186050.019.445715
CTATACT211050.018.8321784
CCTATAC162350.017.833833
GTATAGG164950.017.729021
TAGACTG104550.015.9606975
GTATTAT219800.015.6629231
TTATACT236350.015.5892684
TAATACT238650.015.5784754
GTATTAG139100.015.4857781
GTATATA420650.015.3963621
GTATAGA159300.015.3429641
ATTATAC211150.015.063113
TATACCG100350.014.9705745
TATACTA300000.014.8803495
GTATAAT223750.014.7701091
TATACAG178500.014.4354345
GTATAAG135150.014.1088061
ACTATAC161600.013.88613
TACACCG97450.013.757395
TCTATAC157700.013.6265653
Produced by FastQC (version 0.11.2)