FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l08n02_138.35200000073c87.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l08n02_138.35200000073c87.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57585463
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG348950.020.4952351
CTATACT422900.019.392394
CCTATAC362300.017.5052153
TATACTG344650.017.3391065
TATACCG191350.015.8263465
TATAGGG322850.015.7598212
TATACAG323150.015.2414165
TATACTA590400.015.227025
GTATATA802850.015.2099881
ACTGTGC255450.015.0814268
GTATAAG253750.015.0292251
TAGACTG192100.014.9973665
TTATACT440550.014.7952464
TACACTG267450.014.7360695
TAATACT444200.014.4489114
CCCTATA330900.014.28426552
GTATTAT406900.014.269091
TCTATAC278900.014.250753
GTATTGG350000.014.0753761
GTATAGA291650.013.7283521