FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l08n02_137.35200000073c1d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l08n02_137.35200000073c1d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12841207
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG96250.023.3125255
GTATAGG80250.022.0419961
CTATACT124250.021.5029544
TTATACT158100.019.5151124
CCTATAC92150.019.0365923
TAATACT159100.018.8247224
TACACTG63800.018.255495
GTATTAT130850.018.1697651
ACTGTGC60700.017.9996458
ATACTGT92700.017.8855656
GTATAGA95450.017.1868461
CTGTGCG29900.016.8404779
CTAATAC75500.016.4972883
GTATTAC76800.016.4692361
GTATTGG88150.016.3985081
GTATAAT132200.016.3656391
TAAGACT67800.016.3374564
GTATAAG74350.016.1805231
GGACCGT27300.016.009666
TCTATAC85050.015.9863743
Produced by FastQC (version 0.11.2)