FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l08n02_136.35200000073b2b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l08n02_136.35200000073b2b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39775685
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAAC489520.12307016208520356No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG280000.022.3657861
TATACTG273550.021.6543355
CTATACT343700.021.149534
TTATACT383800.019.4848274
TAATACT390100.019.4138934
ACTGTGT242900.018.2464498
GTATTAT346250.018.1550771
TATACCG140150.018.147815
CCTATAC296950.017.9951633
ACTGTGC187350.017.596658
TAGACTG137350.017.4793875
GTATAAT346050.016.90141
ATAATAC325500.016.8987713
TATACAG252100.016.7268525
GTATTAC200550.016.5496641
TATAGGG248250.016.5301952
ATACTGT282850.016.3257066
TATACTA495950.016.219075
GTATAAG212600.016.0142351
TCTATAC227850.015.9827873
Produced by FastQC (version 0.11.2)