FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l08n02_113.35200000073abf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l08n02_113.35200000073abf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33372535
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG193550.022.5784341
CTATACT260950.020.368864
CCTATAC215050.018.9020483
TATACTG202850.017.9758535
TATAGGG191750.017.582572
TATACCG108600.016.2410165
TAATACT259800.016.21354
GTATTAT247300.016.055871
TATACTA352700.015.918925
CCCTATA202400.015.6706422
GTATATA447900.015.6384291
TTATACT255700.015.2277374
ATAATAC237200.014.9722873
GTATTGG213150.014.9026181
TATACAG192450.014.8959625
GTATAAT249000.014.819511
GTATTAG155450.014.53029251
GTATAAG149500.014.3133991
GTATTAC151500.014.3127691
TAGACTG109100.014.2492555
Produced by FastQC (version 0.11.2)