FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l08n01_220.35100000073b98.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l08n01_220.35100000073b98.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35016453
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG235650.021.2264231
CTATACT293400.021.028154
CCTATAC244450.019.5270253
TATACTG217950.019.263635
TATACCG112050.017.4209825
TATAGGG213850.016.7907752
TATACTA406000.016.6581465
CCCTATA223050.016.3963342
TATACAG207900.016.1969955
TTATACT299500.016.0485464
TAATACT285050.016.0456494
GTATTAT261450.015.9340741
TAGACTG116300.015.681745
ATTATAC261150.015.3501453
ACTGTGC160000.015.1391118
ATAATAC255850.015.1297663
GTATATA528500.014.6417171
ATACTGT241350.014.1491966
ACTATAC189550.014.1073263
GTATAAT266550.013.989651
Produced by FastQC (version 0.11.2)