FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l08n01_138.35100000073c8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l08n01_138.35100000073c8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57585463
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT439800.019.9570854
GTATAGG349650.019.0879271
TATACTG344150.018.3132425
CCTATAC369550.018.2373833
TATACTA601100.016.1343355
TACACCG185500.016.1301615
TATACAG336250.015.4806915
TAGACTG192350.015.4322735
TATACCG187600.015.3926285
TAATACT446700.015.2788674
TTATACT442650.015.0645794
TACACTG267050.014.796975
CCCTATA337100.014.7657392
TATAGGG320950.014.4136572
ACTGTGC251100.014.3941268
GTATTAT406500.014.3266921
TCTATAC286650.014.3157853
GTATATA801200.014.1878861
ATAATAC404550.014.0649423
GTATAAG255850.013.9991581