FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l08n01_136.35100000073b2e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l08n01_136.35100000073b2e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39775685
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAACCCTAAAC494520.1243272114609717No Hit
GGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGG414600.1042345342386938No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG275150.022.2153475
CTATACT353900.021.7011244
GTATAGG276550.020.2176111
TAATACT393200.020.0273654
TTATACT383250.019.8285354
TATACCG144100.019.7427165
TAGACTG137700.019.2461725
ACTGTGC184100.018.8072818
CCTATAC297600.018.7525333
ATACTGT280100.017.9740126
ACTGTGT257200.017.0629168
TACACTG201000.017.0366135
GTATTAT343250.017.0224321
ATTATAC341200.016.9130633
TCTATAC231900.016.8764653
ATAATAC324250.016.8304233
TATACTA511450.016.7245565
ACTGCGC88550.016.4128918
AATACTG230900.016.2086325
TATACTC331000.016.1424185
Produced by FastQC (version 0.11.2)