FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l08n01_113.35100000073ab2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l08n01_113.35100000073ab2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33372535
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT268450.021.2839934
CCTATAC216600.020.436743
GTATAGG195250.020.1697391
TATACTG198150.018.8875035
TATACTA366550.017.4273575
CCCTATA202900.017.2051892
TATAGGG194600.016.0352842
TAATACT266450.015.8109794
TACACTG140000.015.7749275
ACTGTGC145650.015.5219188
TATACAG192900.015.4621485
TTATACT269700.015.4443464
TATACCG108850.015.31514555
ATAATAC243350.015.1064973
GTATTAT247750.015.0136021
GTATATA456900.014.7120121
ATACTGT212500.014.6620226
ATTATAC243700.014.5780763
TCTATAC176800.014.3454153
TAGACTG111500.014.2696195