FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l07n02_82.35200000073cbd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l07n02_82.35200000073cbd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27421880
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT250450.020.594284
TATACTG207400.020.5817225
GTATAGG168700.019.111171
CTGTGCG57300.018.8202849
TTATACT316000.018.7593274
ACTGTGC126050.018.5806038
TAATACT317400.018.2721984
CCTATAC174600.017.5345783
CTAGACT108600.017.202864
TAGACTG90300.016.8452075
TACACTG132600.016.5978415
TATACAG169800.016.2369485
TAGGACT114350.016.2131084
GTATTAT277300.016.1194671
ATACTGT222150.016.081766
GTATAGA205700.015.8353981
CTACACT128900.015.7106654
TGTACTG195500.015.4397785
ACTGCGC47700.015.437228
CTAATAC160250.015.4262043