FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l07n02_6.35200000073ae6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l07n02_6.35200000073ae6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33987718
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG209250.016.7429185
GTATAGG150950.015.0269381
GTATATA451300.014.9942521
TATACAG198400.014.9271025
GTATTGG255200.014.3294951
ACTGTGC144750.014.2438048
CTATACT210250.014.1319034
GTATTAT243050.014.1262471
GTATAAG144750.013.600881
TAATACT262350.013.5361814
CCTATAC146900.013.5278783
TATACCG102800.013.4101625
TTATACT274450.013.3204764
TAGACTG112650.013.2081595
TACACTG153650.013.1797195
GTATAGA184150.013.118291
ATAATAC247450.013.1030423
GTATTAG157950.013.0663811
GTATAAT250400.013.0469081
GTCTTAG102050.012.7213991