FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l07n02_32.35200000073bd8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l07n02_32.35200000073bd8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61629310
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG373850.015.7033915
GTATTGG482450.014.9918921
GTATAGG263350.014.8609311
TATACCG199450.014.8245075
CTATACT375800.014.7119364
GTATATA818950.014.3306961
GTATAAG279050.013.9055341
TATACAG343200.013.8647085
CCTATAC271950.013.6879013
ACTGTGC252700.013.4605348
GTATTAT459950.013.3163371
TTATACT473800.013.2843334
TAATACT476600.013.1564164
GTATTAC272150.012.9476031
ATAATAC438600.012.8660783
GTATTAG292200.012.8077861
GTATAAT448100.012.6921661
TCTATAC305400.012.6401183
TACACTG275100.012.562265
ATTATAC438100.012.4141473