FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l07n02_19.35200000073b6e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l07n02_19.35200000073b6e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30263890
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT241200.019.7270814
GTATAGG180450.019.5252531
TATACTG187700.019.4738065
CCTATAC194950.018.6780263
TAATACT273800.017.8643824
TTATACT264650.017.6917344
GTATTAT246800.016.9154951
ATAATAC236400.016.9111823
TATACAG177750.016.6329635
TATACTA332100.016.4595435
TAGACTG95300.016.3096145
GTATATA467750.016.2543661
ATTATAC238350.016.2542863
TATACCG94850.016.0863135
GTATAAT244900.015.55174351
TCTATAC163250.015.49115853
TACACTG142550.015.4717555
GTATAAG146100.015.296181
GTATTAC143700.015.2207621
CCCTATA180600.015.2175752
Produced by FastQC (version 0.11.2)