FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l07n01_52.35100000073a7f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l07n01_52.35100000073a7f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21882260
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG164450.024.9798365
ATACTGT177550.021.3983676
TTATACT279800.021.0639764
CTATACT198750.020.8604354
TAATACT307850.020.3325824
TAGGACT83950.019.8564994
TACACTG114300.019.4034085
ACTGTGC99100.019.3622478
AGGACTG66150.018.881365
TTACACT168350.018.8160724
TAAGACT121950.018.7318024
AGTACTG118150.018.6907445
TAGACTG65000.018.484795
CTACACT122600.018.4388054
TAGACAG84000.018.4308625
TAAGGCT92950.018.3937134
TATACAG157150.018.2225975
TCTATAC172150.018.1806553
CTACCCT81100.017.9776884
AATACTG158600.017.9661665