FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l07n01_19.35100000073b61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l07n01_19.35100000073b61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30263890
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG184800.020.3794165
CTATACT239600.020.2973864
GTATAGG187000.019.510541
CCTATAC190150.018.8076383
TAATACT268600.017.9114384
TTATACT260400.017.6547414
TATACAG176000.017.593635
TATACCG100800.016.4431675
ATACTGT193600.016.2885556
ATTATAC233650.016.017553
ACTGTGC128900.015.9547758
TAGACTG97500.015.8793795
TATACTA348050.015.7738135
GTATTAT239350.015.7195991
ATAATAC239550.015.6825223
GTATTAG152350.015.6534821
TACACTG142900.015.32107455
TATAGGG182100.015.2314812
TCTATAC157600.015.1581913
TATACTC226650.015.0448985