FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l06n02_215.35200000073c51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l06n02_215.35200000073c51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37861548
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG215150.016.4568825
CTATACT219200.016.2405994
GTATAGG153150.015.55205251
TATACAG203750.015.4649515
GTATATA454300.015.3620891
TTATACT280950.014.8872474
CCTATAC153950.014.541493
GTATTAT262800.014.3092911
GTATAAG156800.014.2501291
TAATACT289100.014.2373964
GTATTGG276200.014.2002911
TATACTA324600.013.8508135
TCTATAC183450.013.8094743
TATACCG111150.013.7682595
TAGACTG116450.013.7538125
ATTATAC248950.013.5936333
GTATAAT259350.013.58311
ACTGTGC148700.013.5775988
ATAATAC265050.013.485213
TACACTG163600.013.450255