FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l06n02_214.35200000073be5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l06n02_214.35200000073be5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72505150
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG504850.019.7033675
CTATACT525500.017.6822664
TAATACT643150.017.5367574
GTATAGG342300.017.1248151
GTATTAT584300.016.9400441
TATACAG465550.016.548185
TTATACT654900.016.191554
ATAATAC580350.016.1915253
GTATATA1154000.015.6877561
GTATAAT592500.015.6143591
GTATTAG373400.015.5584591
GTATAAG354250.015.5540431
ATACTGT523100.015.4214966
GTATTGG655150.015.38384151
GTATAGA431800.015.380961
TATACCG247150.014.9607235
TAGACTG249400.014.8257525
CCTATAC338650.014.8212183
TCTATAC414500.014.7923183
ATTATAC576150.014.7833723
Produced by FastQC (version 0.11.2)