FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l06n02_212.35200000073b7b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l06n02_212.35200000073b7b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28187433
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG172950.019.4558965
GTATAGG147400.018.1584631
CTATACT193650.017.917394
CCTATAC151400.016.6703913
GTATATA390250.016.0317571
GTATTAT204900.015.684581
TATACAG165000.015.3814025
TAATACT222800.015.3598414
TATACCG91500.015.1150825
TAGACTG101250.015.0677575
TTATACT227250.014.9126594
GTATTGG194400.014.6731421
ATAATAC196600.014.6267913
GTATAAT206600.014.3589421
TACACTG131900.014.3048835
TATACTA271100.014.0950025
TCTATAC144450.013.9516473
GTCTTAG83300.013.925571
GTATAAG132900.013.8795351
GTATTAG127200.013.7913691