FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l06n01_215.35100000073c54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l06n01_215.35100000073c54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37861548
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG217050.018.3587175
CTATACT221300.016.4180624
TATACAG201050.016.0873015
TATACCG112100.015.5065685
TAATACT289950.015.3154734
GTATAGG152550.015.1640391
TACACTG169650.014.9215375
GTATATA461400.014.9069591
CCTATAC160000.014.7232083
TTATACT284600.014.6521564
TATACTA329900.014.2957565
GTATTAT267700.014.0709341
GTATAAG157850.013.9326741
TCTATAC180950.013.59602453
ATAATAC264300.013.5492413
GTATTGG275700.013.5248051
TAGACTG115050.013.41642955
TAGACAG124950.013.3416975
CTAATAC169850.013.3101263
GTATAAT263150.013.3033871