FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l06n01_214.35100000073be8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l06n01_214.35100000073be8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72505150
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG512350.020.2081725
TAATACT661100.018.3840794
TTATACT649850.018.1615144
CTATACT528550.017.6659184
TATACAG478500.017.062055
ACTGTGC339900.016.7676378
GTATTAT607750.016.5775381
ATAATAC584650.016.5232753
ATTATAC586100.016.133953
TATACCG243950.015.8085655
GTATAAG352400.015.39341
GTATTAG387150.015.3736221
CCTATAC341550.015.3516813
GTATTGG641000.015.295231
ATACTGT540000.015.0924346
GTATAGG336050.015.0398011
TACACTG365600.015.0171985
GTATATA1181850.014.9193531
GTATAAT593250.014.916931
TAGACTG250450.014.9052315