FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l06n01_212.35100000073b7e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l06n01_212.35100000073b7e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28187433
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG173350.019.4798245
CTATACT197650.019.0793574
GTATAGG151450.017.0934491
CCTATAC150400.016.9892023
TAGACTG97300.016.6448865
TAATACT227900.016.0467264
TATACAG164050.015.8651295
TCTATAC148050.015.7832073
GTATAAG131350.015.5865381
TTATACT227200.015.448144
GTATTAT203350.015.253571
TATACTA276950.015.1253885
ATAATAC210650.015.060993
GTATATA398750.014.8786741
TATACCG90050.014.8732095
ATTATAC199450.014.6922953
TACACTG133950.014.4662755
GTATTAG133600.014.2218481
ATACTGT189700.014.2206266
TACACAG127250.014.2202255