FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l06n01_209.35100000073b04.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l06n01_209.35100000073b04.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35471847
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT302250.021.4177274
GTATAGG224350.020.897381
TATACTG225450.020.3714495
CCTATAC242050.019.9357433
TATACTA422200.018.381445
TTATACT306500.017.8511334
TAATACT316800.017.8404434
CCCTATA233100.017.4208812
TATACAG217450.016.665215
TATAGGG221700.016.1529522
TAGACTG116450.016.1510735
TATACCG111250.016.0948525
GTATTAT282600.015.9848711
ATTATAC275450.015.6734623
TACACTG168050.015.374685
ATAATAC273600.015.3628233
ACTGTGC162500.015.2276578
ATACTGT236300.015.0342486
GTATATA521600.014.9259561
GTAATAC166950.014.1388423