FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l06n01_207.35100000073a8c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l06n01_207.35100000073a8c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86794020
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG585850.018.0699835
TAATACT743750.017.113684
CTATACT590900.016.9028874
TATACCG293750.016.4266855
TTATACT741000.016.3824274
TATACAG535300.015.6507595
GTATAGG413450.015.6250631
GTATTAT680900.015.5500791
ATTATAC669450.015.5085993
ACTGTGC392050.015.421478
ATAATAC690550.015.2273063
GTATATA1288100.014.7508261
GTATTAG430600.014.7048981
TAGACTG299800.014.5902295
TCTATAC468800.014.55826953
TACACTG418200.014.1730935
GTATAAT680350.014.1592881
GTATAGA487000.014.094341
CCTATAC418200.014.0710943
GTATAAG417400.014.0319011