FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l05n02_202.35200000073c37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l05n02_202.35200000073c37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33713427
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG197600.020.89011
CTATACT245100.019.8608264
CCTATAC204850.018.925443
TATAGGG187150.016.7466432
CCCTATA190800.016.2512
TATACTG195150.016.2284265
TATACTA326000.015.6782495
GTATATA417800.015.4947511
TATACAG189650.014.8604045
GTATTAT226500.014.8075311
TAATACT243200.014.712444
ACTGTGC149000.014.4181488
GTATTAG153300.014.3672141
TAGACTG112150.014.14067655
GTATAAG146950.013.9801761
GTATTGG220950.013.9253341
TTATACT247250.013.8145324
TCTATAC162450.013.51182653
TATACCG103300.013.4100095
TACACCG110550.013.3515335