FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l05n02_201.35200000073bcb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l05n02_201.35200000073bcb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40892911
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG239500.016.4568545
GTATTGG390700.016.2421051
GTATAGG165800.015.4190261
TATACCG120100.015.2354115
TATACAG230700.014.9101945
CTATACT246950.014.8975694
GTATATA521850.014.8614271
GTATTAT296750.014.299141
TGCGTTC273650.014.1325079
TACACTG184350.013.9942315
TATTGGG410500.013.7708032
ACTGTGC169700.013.7584998
TTATACT305750.013.6733624
TAATACT306850.013.5464914
GTATTAG194800.013.51606751
CCTATAC167700.013.3721183
TAGACTG132500.013.3048175
GTATAAG177350.013.2832211
GTGCGTT310850.013.19438
GTATAGA212450.013.1129231
Produced by FastQC (version 0.11.2)