FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l05n02_199.35200000073af3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l05n02_199.35200000073af3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21683203
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG133800.017.9555445
GTATAGG92800.015.7521481
CTATACT144450.015.7069564
CCTATAC95300.015.2054083
TAATACT188300.014.9917764
TATACAG131850.014.9608635
TTATACT183850.014.8350314
GTATATA305700.014.7829821
GTATTGG175950.014.4983421
TAGACAG74150.014.4929925
GTATAAG94750.014.369181
GTATAGA121500.014.3510251
ATAATAC166900.014.2902073
TATACCG61800.014.1432545
GTATTAG107500.014.1313931
GTATTAT175850.014.0990991
TACACTG102150.013.9803985
TAGACTG70550.013.8108335
GTATAAT166750.013.5506971
ATTATAC161900.013.3816343
Produced by FastQC (version 0.11.2)