FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l05n02_198.35200000073a96.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l05n02_198.35200000073a96.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35370722
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG216050.016.7608265
GTATAGG151300.015.4113441
TATACAG202900.014.9510675
GTATATA493050.014.8466121
GTATTAT264250.014.7186841
CTATACT220200.014.60187154
ACTGTGC152400.014.4111178
TATACCG101950.014.3388625
GTATAGA194950.014.1420431
GTATTGG279100.014.123871
TTATACT292200.014.1104664
TACACTG165600.013.9337265
CCTATAC155250.013.6794843
GTATTAG166500.013.660051
TAATACT289000.013.6054464
TAGACTG117100.013.5852575
GTATAAG154500.013.5148921
ATTATAC254850.013.105833
GTATAAT258150.013.1045071
TACACCG109850.012.6988195