FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l05n02_197.35200000073a2c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l05n02_197.35200000073a2c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29045412
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT200850.020.0054154
TATACTG162750.019.8436015
GTATAGG161800.019.37311
GTATTAT200550.017.8695011
CCTATAC173550.017.8490093
TAATACT223350.017.5836584
TTATACT223150.016.807234
GTATATA387650.016.726841
ATAATAC201750.016.6022383
TATACTA278650.016.099095
ATTATAC195550.015.8409373
TATACAG160550.015.4437175
CCCTATA157550.015.2250642
TAGACTG91050.015.1639615
TATACCG88150.015.0124725
ACTGTGC128250.015.0032438
GTATAAT201550.014.93590451
GTATAAG131200.014.9322021
GTATTAC126400.014.7431861
GTATAGA147300.014.662551