FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l05n02_195.352000000739da.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l05n02_195.352000000739da.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30256960
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG202200.016.4472835
TAATACT252000.016.0803994
GTATTAG151550.015.7989711
TTATACT247350.015.3008214
GTATTAT236350.015.1451381
GTATAGG132700.015.1260451
TATACCG88650.014.8224525
TATACAG180000.014.7859345
CTATACT191150.014.5995684
GTATAAT229900.014.5098681
GTATATA456050.014.461511
ACTGTGC129200.014.1931358
TGGTCGC314500.014.16706894-95
TCTATAC157050.014.0973393
GTATAAG136100.014.0107631
CGCCGTA113000.013.6963794-95
GTATTGG209900.013.6383371
GTATAGA168100.013.6180461
ATAATAC226250.013.439423
GTATTAC146050.013.1216911