FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l05n01_200.35100000073b55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l05n01_200.35100000073b55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108497452
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTATAC656700.020.9054343
CTATACT800100.020.7580364
GTATAGG578050.020.0569781
TATACTG635450.017.9088485
CCCTATA621600.017.4444622
TATACTA1107200.016.2922155
TACACTG498250.015.5215165
TATACCG326400.015.4233375
TATAGGG579250.015.2587492
TATACAG611600.014.9910875
TAGACTG357800.014.6786615
GTATATA1372300.014.3751331
GTATTGG653200.014.32889651
TAATACT829600.014.1807584
TTATACT824750.014.1716054
ACTGTGC482550.014.1322478
ATAATAC740000.013.766283
TCCCTAT685850.013.7209751
TACACAG461200.013.6981465
GTATTAT742700.013.4998821