FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l05n01_197.35100000073a2f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l05n01_197.35100000073a2f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29045412
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT198950.021.107174
TATACTG164300.020.6377745
TAATACT222050.017.9715394
TTATACT225450.017.971444
GTATAGG163200.017.8440691
CCTATAC169050.016.4098473
TATACTA281150.016.406215
GTATTAT204750.016.3613831
ATAATAC200700.016.1553083
TATACAG162450.015.8295125
GTATATA379900.015.8270031
TATACTC198900.015.3334025
ATTATAC198100.015.2881773
TCTATAC136600.015.0168063
TAGACTG94550.014.9760435
CCCTATA153800.014.7936452
TAGGGGT282000.014.7827364
ATATACT340450.014.5621544
TATATAC388500.014.5168363
GTATTAA188900.014.3922041