FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l04n02_194.35200000073cd7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l04n02_194.35200000073cd7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45179756
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA453100.020.03959394-95
GCCGTAT319550.018.74497894-95
CTATACT211250.016.315114
GTATAGG163200.015.5002021
CCGTATC239750.015.41450794-95
GTATTGG388500.015.384741
TATACTG232500.015.3337375
GTATAAG158300.014.5081531
GTATATA393850.014.04089451
GTATTAT233000.013.9791431
GGTCGCC1072350.013.90697194-95
GTATAGA182100.013.5519981
ACTGTGC178500.013.5461428
TTATACT244400.013.538074
TCGCCGT875650.013.42891894-95
TGGTCGC1112400.013.25681994-95
TATACCG123500.013.2404045
TATACAG204450.013.1827395
TATTGGG397900.013.16902352
TAATACT233100.012.8279454