FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l04n02_191.35200000073c6d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l04n02_191.35200000073c6d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31099966
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG173600.018.570121
CTATACT209500.017.4720654
CCTATAC179350.016.6720753
TATACTG178800.016.0838415
TATACCG96650.015.5412945
TATACTA293900.014.79869655
TAGACTG99750.014.7723895
GTATTGG194250.014.5888141
GTATATA400250.014.58820151
TATAGGG163600.014.3284222
TATACAG165150.013.7578715
GTATTAG134900.013.7463491
ACTGTGC130900.013.7178868
TTATACT225300.013.609314
GTATAAG135550.013.5751981
TAATACT224550.013.5277444
ATAATAC207400.013.4087263
GTATTAT207000.013.3227041
CCCTATA167000.013.1825462
TACACCG99200.013.0334435
Produced by FastQC (version 0.11.2)