FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l04n02_190.35200000073bf2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l04n02_190.35200000073bf2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26225457
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG157400.017.5463315
GTATTAT185150.015.8970661
CTATACT159700.015.5983774
TATACAG148750.015.4669015
GTATATA345900.015.2451261
GTATAGG111350.015.1169121
TAATACT199650.014.8344614
TTATACT193000.014.6312754
GTATTGG230100.014.3621091
ATTATAC174800.014.2238323
ACTGTGC116450.014.1555418
TATACCG83700.013.9709065
GTATAGA130250.013.9090431
CCTATAC110550.013.8899323
TCTATAC129400.013.8871753
ATAATAC185300.013.67439653
GTATAAT185450.013.5124421
TAGACTG83000.013.4014795
GTATTAC119950.013.3988191
GTATAAG114050.013.3831951
Produced by FastQC (version 0.11.2)