FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l04n02_187.35200000073b1e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l04n02_187.35200000073b1e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18852158
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT155000.021.3765954
GTATAGG109400.021.2966231
TATACTG116100.019.938725
CCTATAC119200.019.6612193
TAATACT156600.017.4243894
GTATTAT145300.017.4091681
TATACTA212400.017.1649045
TTATACT161400.016.7589264
CCCTATA116100.016.5045952
ACTGTGC89250.016.446838
TATACAG109900.016.4356335
TAGACTG65000.016.3808025
GTATATA267000.016.3301471
GTATTGG118950.016.0292031
ATTATAC140950.015.9190313
TATAGGG103950.015.9179342
TATACCG59650.015.6984345
ATAATAC140650.015.5812013
TCTATAC103950.015.5487013
GTATAAT145000.015.5104761