FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l04n02_186.35200000073aa3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l04n02_186.35200000073aa3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51260886
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG328100.018.4429825
TTATACT424900.016.8605584
TAATACT435900.016.7186324
TATACAG310400.016.5390665
GTATTAT404700.016.4607181
TATACCG158600.016.424285
CTATACT330200.016.1101074
GTATATA756050.015.6222981
ATAATAC385000.015.4717113
GTATAGG220150.014.9462151
GTATAAG238300.014.7656141
GTATTAC246650.014.6513051
TCTATAC271250.014.5464263
GTATAGA281600.014.5045531
TAGACTG169450.014.3907955
TACACTG244600.014.3614055
GTATTAG253350.014.3013781
ATTATAC373000.014.2871233
TAGACAG173550.014.21515755
GTATTGG375850.014.0554041
Produced by FastQC (version 0.11.2)