FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l04n02_184.35200000073a39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l04n02_184.35200000073a39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31646045
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG176550.015.5618535
CTATACT185200.015.2213824
GTATAGG138500.015.0369891
TATACCG95100.014.1451375
TAGACTG102950.013.7591355
CCTATAC142550.013.6395663
GTATATA374250.013.5435311
ACTGTGC133050.013.3532218
GTATAAG129750.013.0094081
TACACTG139000.012.9606145
TATACAG165000.012.9061075
GTATTGG189250.012.8638381
GTATTAT211650.012.7380031
GTGTAGG130000.012.6186321
TCTATAC139600.012.5656633
TAGACAG104050.012.5629565
ACTATAC148050.012.3301193
TTATACT215750.012.1846954
GTATTAG142900.012.144991
TAATACT228050.012.0903334