FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l04n01_191.35100000073c60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l04n01_191.35100000073c60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31099966
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT214600.019.4324054
GTATAGG171300.019.0805071
CCTATAC180200.018.3714143
TATACTG170700.017.2510855
TATACCG96200.016.0459925
TATACAG170350.015.3069435
TAGACTG101750.015.1240785
CCCTATA167700.015.01092152
TATAGGG167900.014.993042
TATACTA294700.014.8596675
TCTATAC145850.014.7196193
GTATTAT210650.014.59155751
TTATACT225300.014.5672854
GTATATA402250.014.49131
TACACTG140100.014.3742765
ATAATAC208200.014.3722373
ACTGTGC132100.014.3459878
GTATTAG140200.014.2318091
TAATACT228150.014.1563154
GTATAAG133500.013.5582151
Produced by FastQC (version 0.11.2)