FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l04n01_190.35100000073bf5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l04n01_190.35100000073bf5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26225457
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG154250.017.8901215
GTATAGG114950.016.0775431
CTATACT161750.015.8861164
GTATTGG238400.015.8231631
TATACAG145600.015.2341355
TATACCG87100.014.9960495
GTATATA344250.014.6978811
TTATACT198050.014.5812084
TACACTG122550.014.2625375
GTATTAT190700.014.1008341
TAGACTG82500.014.0474715
ACTATAC125100.013.9720283
GTATTAG123400.013.8216011
TATTGGG245550.013.7530362
ACTGTGC116100.013.7049478
ATTATAC176050.013.5976363
TAATACT196650.013.5498264
ATAATAC177250.013.3447993
GTATAGA132900.013.262581
CTACACT125300.013.19146254
Produced by FastQC (version 0.11.2)