FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l04n01_189.35100000073b8b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l04n01_189.35100000073b8b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55897572
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT378850.018.41724
GTATAGG282500.018.0781631
CCTATAC299750.016.9707763
TATACTG336250.016.7526725
TATACAG313750.015.7590035
TAGACTG189500.015.1136665
TATACCG166600.014.910375
TATACTA529150.014.2628665
TTATACT421900.014.0949624
TACACTG248000.014.0191325
ACTGTGC247000.013.9989098
TAATACT423950.013.9483814
GTATTAT379850.013.6951321
TACACCG170800.013.6538565
GTATATA731950.013.5588031
GTATTGG337700.013.53344251
ATAATAC381100.013.5226623
CTACACT250400.013.3728484
CCCTATA278600.013.3150062
GTATTAG245950.013.2701011