FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l04n01_186.35100000073aa6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l04n01_186.35100000073aa6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51260886
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG327200.018.3189565
TTATACT429950.016.7250774
TAATACT438250.016.6034014
CTATACT323300.016.086764
TATACCG156550.015.836975
TATACAG307850.015.5824915
TAGACTG168450.015.5076625
GTATATA756300.015.2140491
GTATTAT399550.015.0174791
GTATAAT400000.015.0005851
ATAATAC400550.014.98833753
ACTGTGC225600.014.9268358
GTATTAG258000.014.8599941
ATTATAC381500.014.8528243
TCTATAC268050.014.3880973
CCTATAC215750.014.0893943
GTATTGG361200.014.0734591
GTATAGA280500.013.9728791
GTATAGG224900.013.8361941
GTATAAG226650.013.8132071
Produced by FastQC (version 0.11.2)