FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l04n01_184.35100000073a3c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l04n01_184.35100000073a3c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31646045
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACT189500.015.640294
TATACTG177450.015.444195
CCTATAC138400.014.2423893
TATACCG99850.014.2229095
GTATAGG139450.014.1721651
TACACTG140300.013.9816275
ACTGTGC134000.013.363028
GTATATA373350.013.348141
GTATAAG132050.013.311431
TATACAG171800.013.2703825
TACACCG102700.012.9494985
TAATACT232300.012.5541884
GTATTAT211350.012.497811
TATACTA262450.012.487285
TCTATAC142950.012.426773
TAGACTG104250.012.3013575
TTATACT220300.012.2678164
TACACAG132800.012.1961185
GTATTAG137050.012.0978391
TAGACAG100400.012.06345755