FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l03n02_rafflesia_totalrna_seed.3520000007398a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l03n02_rafflesia_totalrna_seed.3520000007398a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences172545511
Sequences flagged as poor quality0
Sequence length101
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC60086333.482346753141553Illumina Single End PCR Primer 1 (100% over 50bp)
GGGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG11760910.681612053065814Illumina Single End PCR Primer 1 (100% over 44bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC11337470.6570712813270465Illumina Single End PCR Primer 1 (100% over 49bp)
GGCGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG10434690.6047500128821086Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG9969270.577776259852973Illumina Single End PCR Primer 1 (100% over 44bp)
GGCTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG8900140.5158140567331247Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG8474350.49113708904313363Illumina Single End PCR Primer 1 (100% over 44bp)
GGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG7188980.4166425401817611Illumina Single End PCR Primer 1 (100% over 48bp)
GGGCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG7173330.41573553310233613Illumina Single End PCR Primer 1 (100% over 44bp)
GGCCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG7149480.4143532890867268Illumina Single End PCR Primer 1 (100% over 44bp)
GGCGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG7121540.41273400616026457Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG7015700.4065999723400512Illumina Single End PCR Primer 1 (100% over 45bp)
GGGCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG6823110.3954382794693512Illumina Single End PCR Primer 1 (100% over 44bp)
AGATCGGAAGAGCGTCGTGTCGGGAAAGAGTGTAGATCTCGGTGGTCGCC6254710.3624962459904274Illumina Single End PCR Primer 1 (98% over 50bp)
GTGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG6193550.3589516739151794Illumina Single End PCR Primer 1 (100% over 44bp)
GGGTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG6184790.3584439817735971Illumina Single End PCR Primer 1 (100% over 44bp)
GGCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG6049850.3506234363871686Illumina Single End PCR Primer 1 (100% over 44bp)
GCGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG5984500.3468360298286752Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG5776710.33479340995431633Illumina Single End PCR Primer 1 (100% over 44bp)
GGCAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG5535590.32081912580154Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG5249540.30424089097281704Illumina Single End PCR Primer 1 (100% over 45bp)
GGCCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG5131730.2974131271372224Illumina Single End PCR Primer 1 (100% over 44bp)
TGGGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG4919920.2851375252526854Illumina Single End PCR Primer 1 (100% over 44bp)
GGTGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG4908170.28445654549656757Illumina Single End PCR Primer 1 (100% over 44bp)
GGCGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG4896160.2837604972522293Illumina Single End PCR Primer 1 (100% over 45bp)
GGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT4725980.2738975921546866Illumina Single End PCR Primer 1 (100% over 46bp)
TGGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG4442440.25746482619301525Illumina Single End PCR Primer 1 (100% over 44bp)
GGGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG4420880.256215300785194Illumina Single End PCR Primer 1 (100% over 45bp)
GGGCCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG4204960.24370150087532558Illumina Single End PCR Primer 1 (100% over 44bp)
GGCCGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG4119250.23873411577772083Illumina Single End PCR Primer 1 (100% over 44bp)
GGCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG4100090.23762368410731935Illumina Single End PCR Primer 1 (100% over 45bp)
GCGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT4092720.23719655042199272Illumina Single End PCR Primer 1 (100% over 46bp)
GTGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG4060310.23531820540958612Illumina Single End PCR Primer 1 (100% over 45bp)
GGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT4042480.23428485485200481Illumina Single End PCR Primer 1 (100% over 46bp)
GCGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG3938330.22824876620522455Illumina Single End PCR Primer 1 (100% over 44bp)
GGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT3880830.2249163120795417Illumina Single End PCR Primer 1 (100% over 46bp)
GGCTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG3679850.21326837068511162Illumina Single End PCR Primer 1 (100% over 44bp)
TGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG3602720.20879824569878264Illumina Single End PCR Primer 1 (100% over 48bp)
AAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC3584710.20775446311089482Illumina Single End PCR Primer 1 (100% over 49bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC3570690.20694192386146748Illumina Single End PCR Primer 1 (100% over 49bp)
GTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT3566730.2067124191947248Illumina Single End PCR Primer 1 (100% over 46bp)
GCTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG3539100.2051111025426793Illumina Single End PCR Primer 1 (100% over 45bp)
GGCGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG3525460.2043205864683434Illumina Single End PCR Primer 1 (100% over 44bp)
GGCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG3518740.20393112400356794Illumina Single End PCR Primer 1 (100% over 45bp)
GGCCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG3431410.19886985063320484Illumina Single End PCR Primer 1 (100% over 45bp)
GTGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG3392050.19658871333952002Illumina Single End PCR Primer 1 (100% over 44bp)
AGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG3299440.1912214337468333Illumina Single End PCR Primer 1 (100% over 48bp)
GGCCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG3264730.18920979056939938Illumina Single End PCR Primer 1 (100% over 44bp)
GCGGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG3241650.18787217246121227Illumina Single End PCR Primer 1 (100% over 44bp)
TGGGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG3223110.18679767333964428Illumina Single End PCR Primer 1 (100% over 44bp)
GCGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG3068800.1778545255807901Illumina Single End PCR Primer 1 (100% over 45bp)
GCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT3059590.1773207533634416Illumina Single End PCR Primer 1 (100% over 46bp)
TGGGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG3009450.1744148533658462Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG3005400.17418013268395025Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2976220.17248898465982113Illumina Single End PCR Primer 1 (100% over 44bp)
GGCCATAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2950400.17099256786807976Illumina Single End PCR Primer 1 (100% over 44bp)
GCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG2937340.1702356661136203Illumina Single End PCR Primer 1 (100% over 48bp)
GGCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT2923710.16944572959652365Illumina Single End PCR Primer 1 (100% over 46bp)
GGGCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG2888090.16738134671031807Illumina Single End PCR Primer 1 (100% over 45bp)
GCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT2883000.16708635207554023Illumina Single End PCR Primer 1 (100% over 46bp)
GCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC2864330.166004318709862Illumina Single End PCR Primer 1 (100% over 47bp)
TGCGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2863010.16592781715428112Illumina Single End PCR Primer 1 (100% over 44bp)
GCTGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2836880.164413434088123Illumina Single End PCR Primer 1 (100% over 44bp)
GCCTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2830580.164048313027396Illumina Single End PCR Primer 1 (100% over 44bp)
GGGCCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2818230.16333255983692327Illumina Single End PCR Primer 1 (100% over 44bp)
GGCAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2801410.16235774456050614Illumina Single End PCR Primer 1 (100% over 44bp)
GCCAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2796500.1620731819560348Illumina Single End PCR Primer 1 (100% over 44bp)
AGGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2727350.15806554364662667Illumina Single End PCR Primer 1 (100% over 44bp)
GCAGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2675860.1550814034217326Illumina Single End PCR Primer 1 (100% over 44bp)
AGGGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2664280.15441027613868205Illumina Single End PCR Primer 1 (100% over 44bp)
GTGCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2656900.15398256289611614Illumina Single End PCR Primer 1 (100% over 44bp)
GGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG2625610.15216912829450543Illumina Single End PCR Primer 1 (100% over 45bp)
TGGGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2615500.15158319592562453Illumina Single End PCR Primer 1 (100% over 44bp)
GCGTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2588340.15000911846382373Illumina Single End PCR Primer 1 (100% over 44bp)
GGGGCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2553510.1479905205995188Illumina Single End PCR Primer 1 (100% over 44bp)
GGGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG2517890.14592613771331323Illumina Single End PCR Primer 1 (100% over 45bp)
GCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG2511100.14553261834786302Illumina Single End PCR Primer 1 (100% over 45bp)
GCTGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2499430.14485627504965923Illumina Single End PCR Primer 1 (100% over 44bp)
GCCGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2475250.14345490564515467Illumina Single End PCR Primer 1 (100% over 44bp)
GGGCCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2459210.14252529583339899Illumina Single End PCR Primer 1 (100% over 44bp)
GCCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG2424110.14049104992363434Illumina Single End PCR Primer 1 (100% over 45bp)
GGCGTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT2422810.14041570748253196Illumina Single End PCR Primer 1 (100% over 43bp)
TGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG2351300.13627129366466104Illumina Single End PCR Primer 1 (100% over 45bp)
AGCGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2341580.13570796402811083Illumina Single End PCR Primer 1 (100% over 44bp)
GGTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG2298110.13318862870909462Illumina Single End PCR Primer 1 (100% over 45bp)
GGGCAGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2222370.1287990621790213Illumina Single End PCR Primer 1 (100% over 44bp)
GCCAGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2221740.12876255007294857Illumina Single End PCR Primer 1 (100% over 44bp)
TGGGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG2217410.1285116017883537Illumina Single End PCR Primer 1 (100% over 45bp)
GGGCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2214090.12831918878492296Illumina Single End PCR Primer 1 (100% over 44bp)
GCCGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2198650.1274243524075222Illumina Single End PCR Primer 1 (100% over 44bp)
GGTGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2169000.12570596519314836Illumina Single End PCR Primer 1 (100% over 44bp)
GTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC2166980.12558889463082004Illumina Single End PCR Primer 1 (100% over 47bp)
GGCCTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT2161270.12525796744720874Illumina Single End PCR Primer 1 (100% over 43bp)
TGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC2145340.1243347327650848Illumina Single End PCR Primer 1 (100% over 47bp)
AGGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG2132650.1235992746284776Illumina Single End PCR Primer 1 (100% over 45bp)
GGCATGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2114540.12254969646819731Illumina Single End PCR Primer 1 (100% over 44bp)
GGGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2099300.12166645123558155Illumina Single End PCR Primer 1 (100% over 44bp)
GCGGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2090320.12114600883473578Illumina Single End PCR Primer 1 (100% over 44bp)
AGCTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2075740.12030101437991046Illumina Single End PCR Primer 1 (100% over 44bp)
GGCCTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2074980.12025696802972752Illumina Single End PCR Primer 1 (100% over 44bp)
GCAGGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2072770.12012888587985347Illumina Single End PCR Primer 1 (100% over 44bp)
GGCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTC2064520.11965075115747288Illumina Single End PCR Primer 1 (100% over 47bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG2057490.1192433224182807Illumina Single End PCR Primer 1 (100% over 48bp)
AGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG2055990.11915638883239334Illumina Single End PCR Primer 1 (100% over 45bp)
TGCTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG2041170.11829748500382603Illumina Single End PCR Primer 1 (100% over 44bp)
GGGCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG2038500.11814274322094649Illumina Single End PCR Primer 1 (100% over 45bp)
GCGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG2037020.11805696874953762Illumina Single End PCR Primer 1 (100% over 45bp)
GTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG2011120.11655591549988224Illumina Single End PCR Primer 1 (100% over 48bp)
GGCCAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG1999000.11585349212591223Illumina Single End PCR Primer 1 (100% over 45bp)
GGCGGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT1983870.1149766220229282Illumina Single End PCR Primer 1 (100% over 43bp)
GCCTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1983310.11494416681753024Illumina Single End PCR Primer 1 (100% over 44bp)
GTGGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1976500.11454948833760155Illumina Single End PCR Primer 1 (100% over 44bp)
GTCTGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1966850.11399021560172609Illumina Single End PCR Primer 1 (100% over 44bp)
GGCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT1944130.11267346155415194Illumina Single End PCR Primer 1 (100% over 46bp)
GCGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1932130.11197799286705291Illumina Single End PCR Primer 1 (100% over 44bp)
TGGCCTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1910770.11074005860401666Illumina Single End PCR Primer 1 (100% over 44bp)
GTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT1896660.10992230333943605Illumina Single End PCR Primer 1 (100% over 46bp)
GGCCAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGT1870540.10840849983051717Illumina Single End PCR Primer 1 (100% over 46bp)
GGCCGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT1866210.10815755154592228Illumina Single End PCR Primer 1 (100% over 43bp)
GCAGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG1863330.10799063906101851Illumina Single End PCR Primer 1 (100% over 45bp)
AGCCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1852430.10735892167023689Illumina Single End PCR Primer 1 (100% over 44bp)
TGCCTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1844670.10690918525257953Illumina Single End PCR Primer 1 (100% over 44bp)
GGGCGGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGT1761980.10211682644122803Illumina Single End PCR Primer 1 (100% over 43bp)
AGCCGGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTG1752340.10155813326259182Illumina Single End PCR Primer 1 (100% over 44bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC1747500.1012776275587952Illumina Single End PCR Primer 1 (100% over 49bp)
GGGTTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGG1744510.1011043399442597Illumina Single End PCR Primer 1 (100% over 45bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTTA500350.079.828371
GGGGGTA1488500.079.722431
GGGGCTA1384750.077.980811
GGCGGTA955400.075.535581
TGGGGCA733800.074.799331
GGCCTGA1128550.074.562011
AGCGGGA358550.074.301371
AGCCGGA279950.074.283151
GGCGGGA1629150.074.181531
AGGGGCA452900.073.921731
GGCGTGA1083300.073.2458651
GGCAGTA437350.072.964291
ACGGGCA247250.072.748331
GGGGCTG270000.072.708441
GCGGGTA1048600.071.8356861
GTGGGTA591300.070.708161
GTGCGGA426050.070.560571
GGCTGGA1384550.070.207631
GGCCTGG359800.069.090291
GGCAGGA876250.068.71351
Produced by FastQC (version 0.11.2)