FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l02n02_reseq-total_heat_1h_rep3_shoot.352000000739e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l02n02_reseq-total_heat_1h_rep3_shoot.352000000739e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53566052
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA6023401.1244808558973136No Hit
GCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG5619471.049073021099259No Hit
GCCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG4824980.9007533353400771No Hit
CTCTAGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3907250.729426540526078No Hit
GCCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG3419710.6384099391905903No Hit
CTCTATAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3235160.6039571480832674No Hit
CTCTGAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3092210.5772704697370641No Hit
TGACCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT2950890.5508880885976066No Hit
CTCTCAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA2907030.5427000668259069No Hit
CTCTACAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA2791400.5211136336872466No Hit
CTCTTAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA2648090.49435974859599513No Hit
GCCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2476020.462236791317008No Hit
GCCGTAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2412720.4504196053127081No Hit
ACCGGCTCGAACTCGAATTTGATCGCTTTCCATATTTCACAAGCTGCGGC2407950.4495291159408201No Hit
CCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT2029990.37896950105637806No Hit
CGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA1972350.3682089544325574No Hit
GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT1767160.3299029766091405No Hit
GCCGTGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1685980.3147478556007824No Hit
TGACCGAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT1528490.28534677149624543No Hit
TGGTTTATTCAAATTGCAAGGACTCCCAAGCACACGTATTAACTAGAAAG1479090.2761245125924158No Hit
CTCTGGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1459750.2725140169001068No Hit
TCAAAAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA1349100.251857277067946No Hit
GTCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1295920.24192934734111074No Hit
TGGCCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT1245260.2324718648296126No Hit
TGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA1189630.22208655586564416No Hit
GCTGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1184070.22104858502545607No Hit
CCCCCAGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG1094580.20434210831890318No Hit
CTCCAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1014520.18939607496180605No Hit
GGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA1005530.18771777318963137No Hit
CCCCCGGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG954430.17817814910085217No Hit
CTCGAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA943100.17606300348586454No Hit
GCTGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG920930.1719241881033159No Hit
CCGCAAAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT903120.16859932107746153No Hit
CTCAAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA899230.16787311486013567No Hit
CCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT867770.1619999920845389No Hit
CCGCATAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT865970.16166395835929817No Hit
CCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT840020.15681947215374392No Hit
CCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT806070.1504815027248975No Hit
CCCCCCGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG781050.14581063394405097No Hit
AGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA780930.1457882316957016No Hit
GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG747710.13958654261098055No Hit
ATGGTCATTGAGGGCCTCCTAAAAGGATCTACTAAATTCATCGAGTTGTT726460.13561947779911054No Hit
GTCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG723810.13512476148139496No Hit
GCCGGCTCGAACTCGAATTTGATCGCTTTCCATATTTCACAAGCTGCGGC699400.1305677707963245No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC690320.1288726673378878Illumina Single End PCR Primer 1 (100% over 50bp)
CCCCCTGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG679690.12688820150493824No Hit
CCGGGGTGTAGTAAGTCAATTTATAATCCTTAACACCAGCTTTAAATCCA668840.12486266488334814No Hit
TCAAAGATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA648760.12111402199288461No Hit
GCCCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT623510.11640021556936844No Hit
CTTCAAAAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATG576930.10770440950174935No Hit
GCCGTTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG554090.10344051489924999No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCGG664350.075.824161
CCGCGGA746850.075.81592
TGACCGA221050.074.620911
TTGCAGT5527000.073.521079
ATTGCAG5538100.073.401958
CCCGGAC191100.072.0501253
CGGAAGT1113200.071.976575
CTCTACA409600.071.814471
TAATTGC659000.069.751786
GCGGAAG1118600.069.3663254
GAATTGC1184900.069.232596
CTCTATA492200.068.628611
CGCGGAA973000.068.0725863
CGGACAT246250.067.970415
CTATAAT515300.067.948133
AATCTGT1347400.067.4674457
CTCTAGA618650.066.149911
GACCGAA252000.066.057882
GGACATA307250.065.921236
CGAATCT338550.065.27355
Produced by FastQC (version 0.11.2)