FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l02n02_reseq-total_heat_1h_rep1_shoot.35200000073997.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l02n02_reseq-total_heat_1h_rep1_shoot.35200000073997.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66104855
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG6974241.055026896284698No Hit
CTCTAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA6832911.0336472260622915No Hit
GCCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG6085870.9206388849956634No Hit
CTCTAGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA4572090.6916420889207002No Hit
GCCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG4383300.66308291577071No Hit
CTCTATAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3837420.5805050173697529No Hit
CTCTGAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3614350.5467601434115543No Hit
CTCTCAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3546970.5365672460820011No Hit
TGACCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT3519660.5324359307648432No Hit
GCCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG3365480.5091123791134554No Hit
CTCTACAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3345070.5060248600499918No Hit
GCCGTAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG3184930.4817997104751232No Hit
CTCTTAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3170300.47958655986765264No Hit
CGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA2600170.39334024709682824No Hit
CCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT2561500.3874904498315593No Hit
GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT2385800.36091146406720054No Hit
GCCGTGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2330340.35252176258460893No Hit
ACCGGCTCGAACTCGAATTTGATCGCTTTCCATATTTCACAAGCTGCGGC2318750.3507684874280414No Hit
TCAAAAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA1946690.29448517813101627No Hit
TGACCGAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT1901760.2876884004964537No Hit
TGGTTTATTCAAATTGCAAGGACTCCCAAGCACACGTATTAACTAGAAAG1848610.2796481438466206No Hit
CTCTGGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1798440.27205868615852796No Hit
GTCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1701160.2573426717296332No Hit
TGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA1618230.24479745095878358No Hit
GCTGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1590370.24058293449096893No Hit
TGGCCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT1560280.23603107517594585No Hit
GGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA1368170.20696966962562732No Hit
CTCCAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1301270.19684938420937462No Hit
GCTGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1252260.18943540531175812No Hit
CCGCAAAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT1196680.18102755085084146No Hit
CCGCATAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT1153180.17444709620798654No Hit
CCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT1140620.1725470844766243No Hit
CTCGAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1122720.16983926520979437No Hit
GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG1120280.16947015465051699No Hit
CCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT1118310.1691721432563463No Hit
CTCAAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1109140.16778495316266862No Hit
CCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT1104250.1670452192959201No Hit
AGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA1058390.1601077560793379No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC1047190.15841347810232698Illumina Single End PCR Primer 1 (100% over 50bp)
GTCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG984590.14894367440939096No Hit
CCCCCAGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG939250.14208487409888426No Hit
TCAAAGATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA921380.1393815930766356No Hit
GGAGGGGCAGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA900590.1362365895818091No Hit
GCCCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT864580.13078918333608022No Hit
CCCCCGGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG818950.1238865133279545No Hit
CTTCAAAAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATG810400.12259311362228993No Hit
GCCGTTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG782050.11830447249298105No Hit
GCCGAAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG729960.11042456715168651No Hit
GCCGGCTCGAACTCGAATTTGATCGCTTTCCATATTTCACAAGCTGCGGC718730.10872575093009432No Hit
GTCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA712190.10773641361137545No Hit
CCCCCCGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG700010.10589388631137607No Hit
ATGGTCATTGAGGGCCTCCTAAAAGGATCTACTAAATTCATCGAGTTGTT697750.10555200521958637No Hit
CTCTCGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA681100.10303327947697638No Hit
CCGCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGTA680410.10292889985160697No Hit
GCCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA663350.10034815143305284No Hit
TCAAGAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA661070.1000032448448756No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGGA955450.075.408042
GCCGCGG847300.074.7067951
TGACCGA275600.073.874131
CGGAAGT1438200.072.66715
ATTGCAG6973750.071.680738
TTGCAGT6979100.071.59729
CTCTACA499650.071.040141
CGCGGAA1194050.071.018413
CTCTATA570100.070.407791
TAATTGC837150.069.0819856
GCGGAAG1461900.068.975074
GAATTGC1453300.068.4416666
CTATAAT622500.067.951743
AATCTGT1755200.066.630197
CGAATCT435550.066.613115
CTCTAGA722500.066.096561
CCGTGGA377050.065.4936142
CGTGGAA394100.065.256083
CCCGGAC189100.065.096573
CTACAAT563750.065.0006563
Produced by FastQC (version 0.11.2)