FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l02n02_reseq-total_drought_1h_rep3_shoot.35200000073acc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l02n02_reseq-total_drought_1h_rep3_shoot.35200000073acc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121959519
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG10605100.869559021465147No Hit
CTCTAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA10232100.8389751028781935No Hit
GCCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG9241190.7577260123500488No Hit
GCCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG6799870.5575513953937454No Hit
CTCTAGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA6542720.5364665303411044No Hit
CTCTATAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA5578420.4573993113239484No Hit
CTCTGAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA5424200.4447541319017501No Hit
GCCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG5330070.43703599716558406No Hit
CTCTCAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA5257760.431106980669545No Hit
TGACCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT5183870.42504841299021523No Hit
CTCTACAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA4886440.4006608127078625No Hit
GCCGTAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG4729110.38776063064007327No Hit
CTCTTAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA4631660.37977027443015743No Hit
CGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA3881010.3182211632041612No Hit
CCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT3860660.31655257676114645No Hit
GCCGTGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG3638920.298371134113771No Hit
ACCGGCTCGAACTCGAATTTGATCGCTTTCCATATTTCACAAGCTGCGGC2914910.2390063542313577No Hit
GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT2867870.23514933672376978No Hit
TGACCGAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT2773500.22741152332685075No Hit
GTCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2598460.2130592200843298No Hit
CTCTGGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA2597060.21294442789660395No Hit
TCAAAAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA2498690.20487863682046828No Hit
TGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA2401580.19691615871328585No Hit
GCTGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2387850.19579037532937463No Hit
TGGTTTATTCAAATTGCAAGGACTCCCAAGCACACGTATTAACTAGAAAG2354200.19303126310296453No Hit
TGGCCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT2256390.18501138890191915No Hit
GGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA2028940.1663617581174619No Hit
GCTGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1882530.15435695511393416No Hit
CTCCAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1868170.15317951524554635No Hit
ATCCCCATCACGATGAAATTTCCCAAGATTACCCGGGCCTGTCGGCCAAG1766090.14480952487193724No Hit
CCGCAAAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT1738110.1425153210058167No Hit
CCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT1737190.14243988613959685No Hit
CCGCATAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT1699600.13935771589915832No Hit
CTCGAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1683810.13806302401045056No Hit
CCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT1659520.1360713795534074No Hit
CCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT1642910.13470945224046021No Hit
CTCAAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA1617060.13258989648852257No Hit
GCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAAT1574430.129094474372271No Hit
AGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA1573320.1290034605662884No Hit
GTCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG1572330.12892228609068226No Hit
GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG1318680.1081244015073559No Hit
GCCCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT1261570.10344170019234006No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGGA1474600.074.177162
ATTGCAG9809350.072.1807258
TTGCAGT9817450.072.116739
CGGAAGT2307800.069.396555
GAATTGC2056550.067.219636
CGCGGAA1958650.066.671693
GCCGTGG587350.065.992241
AATCTGT2500200.065.797097
GCGGAAG2368200.065.1796954
TAATTGC1221500.064.758476
GCCGCGG1513000.064.130681
CTCTACA792700.063.6786581
GCCGCTG1137700.063.170521
CGCTGAA1269800.062.798563
TGACCGA477850.062.3429371
GGAAGTA3343150.062.1598326
CTCTATA925000.062.0892261
CTATAAT971050.061.9636463
CGAATCT655750.061.796545
CCGCTGA1231750.061.2362482
Produced by FastQC (version 0.11.2)