FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l02n02_reseq-total_drought_1h_rep2_shoot.35200000073a53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l02n02_reseq-total_drought_1h_rep2_shoot.35200000073a53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216845
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG19370.8932647743780119No Hit
CTCTAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA17470.8056445848417072No Hit
GCCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG15960.7360095921049598No Hit
GCCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG11880.5478567640480527No Hit
CTCTAGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA11860.5469344462634601No Hit
CTCTATAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA10000.46115889229634066No Hit
CTCTGAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA9420.43441167654315294No Hit
TGACCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT9300.42887776983559683No Hit
CTCTCAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA9290.4284166109433005No Hit
GCCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG8770.4044363485438908No Hit
GCCGTAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG8630.397980124051742No Hit
CTCTTAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA8260.38091724503677743No Hit
CTCTACAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA8230.3795337683598884No Hit
CGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA6850.31589384122299335No Hit
CCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT6360.2932970555004727No Hit
GCCTGGAATACAATCATAAAGTTGAAAGTACCAGATATTCCTAAAGGCAT6340.29237473771588No Hit
ACCGGCTCGAACTCGAATTTGATCGCTTTCCATATTTCACAAGCTGCGGC6030.2780788120546935No Hit
GCCGTGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG5630.2596324563628398No Hit
TGACCGAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT5100.23519103507113376No Hit
TCAAAAATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA5050.23288524060965207No Hit
GTCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG4800.22135626830224356No Hit
CTCTGGAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA4720.21766699716387283No Hit
TGGTTTATTCAAATTGCAAGGACTCCCAAGCACACGTATTAACTAGAAAG4580.21121077267172406No Hit
TGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA4480.20659918374876063No Hit
GCTGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG4030.1858470335954253No Hit
TGGCCAAATCTGTAACCCTCATTAGCAGATTCGTTTTCAGTGGTTTCCCT3970.18308008024164726No Hit
CTCCAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA3350.15448822891927413No Hit
GGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA3350.15448822891927413No Hit
CCGCAAAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT3320.15310475224238512No Hit
CCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT3150.14526505107334733No Hit
CCGCTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT3090.14249809771956928No Hit
GCTGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG3070.1415757799349766No Hit
CCGCATAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT3020.1392699854734949No Hit
GCGGTCAATAAGGTAGGGATCATCAAAACACCGAACCATCCGATGTAAAG2970.13696419101201318No Hit
AGCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA2700.12451290092001198No Hit
CCGCCGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT2700.12451290092001198No Hit
CTCGAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA2690.12405174202771564No Hit
CTCAAAAATTGCAGTCATGGTAAGATCTTGGTTTATTCAAATTGCAAGGA2690.12405174202771564No Hit
TCAAAGATTGCTATATGTTAGCAGAAAAGCCTTATCCATTAAGAGATGGA2640.12174594756623396No Hit
CCCCCAGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG2400.11067813415112178No Hit
GTCGCGGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2360.10883349858193642No Hit
GCCGTTGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAG2330.10745002190504739No Hit
GTCCGCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAA2270.10468306855126934No Hit
GCCCAGAAGTAGGAATAATGGCACCAGAGATAATATTGTTTCCGTAAAGT2240.10329959187438031No Hit
GGAGGGGCAGCGATGAAGGCGATAATAAATACAGAAGTTGCGGTCAATAA2240.10329959187438031No Hit
CCCCCGGACATACGCAATGCTTTAGCTAATACACGGAAATGCATACCATG2210.10191611519749129No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC2200.10145495630519495Illumina Single End PCR Primer 1 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACAT650.080.408825
ACCGAAT950.080.0425643
CCCGGAC506.366463E-1176.0404363
TGACCGA950.075.03991
GACCGAA1050.072.419462
CTTAAAT1650.072.007993
CCGGGAC200.002067432371.287912
CGAATCT1200.071.2714545
ATTGCAG17650.071.036778
TTGCAGT17700.070.8361059
GCGGAAG4000.070.099784
CTATAAT1900.070.037243
TAATTGC2650.069.8944556
CGCGGAA3500.069.2511143
CGGAAGT4300.068.5089955
GCCGTAG1050.067.893241
ACGCACT505.520633E-966.535383
GAATTGC3650.066.359236
CTCTATA1900.065.0345841
GGACATA950.064.9895866
Produced by FastQC (version 0.11.2)