FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l02n01_reseq-total_heat_1h_rep3_shoot.351000000739ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l02n01_reseq-total_heat_1h_rep3_shoot.351000000739ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53566052
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTT30442985.683259987127668No Hit
TTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG12062982.2519822816137354No Hit
TACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTC8789441.6408601477667237No Hit
GACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCT6573281.227135425250306No Hit
CTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCC4487640.8377768815219013No Hit
GCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAA3973130.7417253748698895No Hit
TGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTC2994470.5590238384564911No Hit
AGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT2511910.46893693042750284No Hit
ACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCC2399750.44799829563694554No Hit
GCAACTGGATAACTAGCACCGAAAACCGTCTTTACATCGGATGGTTCGGT2350340.43877416987908685No Hit
AGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTCT2284410.4264660012651297No Hit
GCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTT2124310.3965776682589936No Hit
ACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTG2036100.38011014886816746No Hit
ATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATA1872010.3494769411044144No Hit
CATGCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAG1858650.3469828241215164No Hit
ACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCG1553070.28993549869981083No Hit
CCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTG1373910.25648894191418103No Hit
CCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTA1345980.251274818610862No Hit
CCACAGCCTAATCCTTATTTTATTCCTACAAATAGAACATAGCCATACGA1312510.24502645817541302No Hit
TGCACTTTTACCCAATTTGGGAAGCTGCATCTGTTGATGAGTGGTTATAC1296670.24206936139329438No Hit
CACAAGGATGAAATATCAGTAATTTTCTATTTACTGGTTGATCCCATCTT1147440.2142102987168067No Hit
TGCTCGTGAAGGTAATGAAATTATCCGATCAGCTTGCAAATGGAGTCCTG1121570.20938074734348538No Hit
AAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCA1056740.19727793267273083No Hit
ACTTAACCGGGGGATTCACCGCAAATACTAGTTTGGCTCATTATTGCCGC1024820.1913189346117948No Hit
ATCGAGTAGACCCTGTCGTTGTGAGAATTCTTAATTCATGAGTTGTAGGG1017780.1900046693752976No Hit
AGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAA1014400.18937367271345665No Hit
CAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT1003920.1874172096909438No Hit
GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCATAACATCCTTAT986160.18410167693523502No Hit
CAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGT954050.1781072086477458No Hit
GTATTGCAATTTGGTGGAGGAACTTTAGGACATCCTTGGGGTAATGCACC930090.1736342263939855No Hit
TAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT859970.16054384594182897No Hit
GATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCG855490.15970749533678533No Hit
CCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTAGAT842190.15722457947806195No Hit
AAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTC813170.15180696908556934No Hit
TTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAA800060.14935952345339917No Hit
GTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTAGATATTGATGGTAT793800.148190872831173No Hit
GTAGTTGATAGCCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAA786700.14686540647050114No Hit
GCTAATGAGGGTTACAGATTTGGTCAAGAGGAAGAAACTTATAATATTGT764800.1427769961467386No Hit
GACTACTTAACCGGGGGATTCACCGCAAATACTAGTTTGGCTCATTATTG761600.1421796028574217No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATG721110.13462071089353383TruSeq Adapter, Index 10 (100% over 49bp)
ATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTATTCGGCGGTTCCCT720400.13448816425746665No Hit
AAACGGATTCAATTTCAACCAATCTGTAGTTGATAGCCAAGGTCGCGTTA716250.1337134198353838No Hit
ACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTAGA713580.13321496980961No Hit
TGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTA691730.12913589375599308No Hit
GTAGCGGGTATAGTTTAGTGGTAAAAGTGTGATTCGTTCTATTAATAACT689090.12864304429230664No Hit
TGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATA678900.12674072003663814No Hit
GGTTCTTTACTTTACGGAAACAATATTATCTCTGGTGCCATTATTCCTAC659390.12309848782583417No Hit
ATCCACGCTGGTACAGTAGTAGGTAAGTTAGAAGGGGAACGCGAAATGAC639580.11940024999415676No Hit
ACCACCAGAGCACAAGAAAGCATAAGCAACACAAGGGATTAACTCATCAC638630.1192228988613908No Hit
CAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGC618990.11555639754820834No Hit
CTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGC596310.11132237261017482No Hit
AGCCGAGCCATTCATTCCTTTCTATGAAAGAAAGATAAGGGGACAGAACA592770.110661506283868No Hit
GGGTATGCGTCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTG583520.10893466630693635No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAAGC3934550.078.389171
AAGCCTT4217200.073.745114
AGCCTTC4246400.073.411155
CCTTCTA4336400.071.865787
CTTCTAT4462250.069.8955158
GCCTTCT4532900.068.861876
TTCTATT5477500.056.9361579
CACAGCC281450.051.787572
CCACAGC324000.049.509871
ACAGCCT300750.048.4957963
CGAGTAG219300.048.380953
GTAGACC230750.046.8589826
CCTAATC317550.045.685117
TAGACCC237500.045.507637
CAGCCTA323150.045.177474
AGCCTAA325000.044.8576245
AGTAGAC258400.041.772285
ACAAGCC7646450.040.8288082
CTAATCC362750.039.927838
CAAGCCT7890650.039.725343
Produced by FastQC (version 0.11.2)