FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l02n01_reseq-total_heat_1h_rep1_shoot.3510000007399a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l02n01_reseq-total_heat_1h_rep1_shoot.3510000007399a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66104855
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTT41715466.3104986766856985No Hit
TACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTC14419062.1812406970713423No Hit
TTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG13080031.9786791756823308No Hit
GACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCT11473381.7356334871319208No Hit
GCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAA6166780.9328785306313734No Hit
CTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCC5065510.7662841102971938No Hit
TGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTC4774860.7223160840455667No Hit
GCAACTGGATAACTAGCACCGAAAACCGTCTTTACATCGGATGGTTCGGT3596940.5441264488062185No Hit
ACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTG3471690.5251792776793777No Hit
GCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTT3380710.5114162946125514No Hit
CCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTA3197560.48371031144384175No Hit
AGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTCT3049080.461249026262897No Hit
CCACAGCCTAATCCTTATTTTATTCCTACAAATAGAACATAGCCATACGA2849780.4310999547612653No Hit
AGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT2325580.3518016944443793No Hit
ACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCC2242480.33923075695423577No Hit
ACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCG2090580.31625211189102526No Hit
ATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATA2019470.3054949594851997No Hit
GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCATAACATCCTTAT1942310.2938225944221495No Hit
TGCACTTTTACCCAATTTGGGAAGCTGCATCTGTTGATGAGTGGTTATAC1668470.2523974978842326No Hit
CCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTG1651980.24990297611272275No Hit
TGCTCGTGAAGGTAATGAAATTATCCGATCAGCTTGCAAATGGAGTCCTG1415220.21408714987726699No Hit
GGTTCTTTACTTTACGGAAACAATATTATCTCTGGTGCCATTATTCCTAC1346740.20372785024640022No Hit
GTAGTTGATAGCCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAA1336720.20221207655625292No Hit
CAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT1304360.1973168234012464No Hit
CACAAGGATGAAATATCAGTAATTTTCTATTTACTGGTTGATCCCATCTT1299950.19664970144779836No Hit
AAACGGATTCAATTTCAACCAATCTGTAGTTGATAGCCAAGGTCGCGTTA1290220.1951777974552701No Hit
CCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTAGAT1219250.1844418235241572No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATG1147270.17355306202547452TruSeq Adapter, Index 9 (100% over 49bp)
TAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT1145250.17324748689033506No Hit
ATCGAGTAGACCCTGTCGTTGTGAGAATTCTTAATTCATGAGTTGTAGGG1115820.16879546895610012No Hit
GATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCG1095580.1657336666119304No Hit
AAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCA1089890.1648729128896811No Hit
AGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAA1084240.16401821016020685No Hit
GTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTAGATATTGATGGTAT1067540.16149192067662804No Hit
TGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATA1063570.16089135964370546No Hit
CAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGT1057900.16003363141784366No Hit
GTATTGCAATTTGGTGGAGGAACTTTAGGACATCCTTGGGGTAATGCACC1040290.15736968184863276No Hit
CAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGC1012820.15321416256037473No Hit
GCTAATGAGGGTTACAGATTTGGTCAAGAGGAAGAAACTTATAATATTGT968590.14652327729937537No Hit
ACCACCAGAGCACAAGAAAGCATAAGCAACACAAGGGATTAACTCATCAC913200.13814416505414012No Hit
GATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATAT907350.13725920736079067No Hit
CTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGC897710.13580091810200628No Hit
TTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAA880240.133158147007508No Hit
TGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTA846130.1279981629185935No Hit
AAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTC841060.1272311995843573No Hit
ATGCATCCATTTCACATGTTAGGTGTAGCTGGTGTATTCGGCGGTTCCCT840110.12708748850595014No Hit
ACTTAACCGGGGGATTCACCGCAAATACTAGTTTGGCTCATTATTGCCGC836880.12659887083936575No Hit
AAGCAAAGCGAGAAAAGAAGCCAGCAATCGAAAGCCCAAGCTTCCAAAAA831180.12573660436892267No Hit
GTAGCGGGTATAGTTTAGTGGTAAAAGTGTGATTCGTTCTATTAATAACT817620.12368531781818445No Hit
CACCACCAGAGCACAAGAAAGCATAAGCAACACAAGGGATTAACTCATCA764870.11570557109610179No Hit
GACTACTTAACCGGGGGATTCACCGCAAATACTAGTTTGGCTCATTATTG761910.11525779763074889No Hit
GGGTATGCGTCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTG752010.11376017691892676No Hit
ACTGCTGTTTTCTTGATTTACCCTATTGGTCAAGGAAGTTTCTCTGATGG750860.11358621087664439No Hit
ACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTAGA733170.1109101593218834No Hit
ATGGATGAAGATACAAAAAAATTTATTTCCAATCGTAACTAAATCTTCTT727050.10998435742730242No Hit
TCTGCAACTGGATAACTAGCACCGAAAACCGTCTTTACATCGGATGGTTC719570.10885282177837013No Hit
GACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTAG707210.10698306501088309No Hit
ATCCACGCTGGTACAGTAGTAGGTAAGTTAGAAGGGGAACGCGAAATGAC682530.10324960246868405No Hit
AGCCGAGCCATTCATTCCTTTCTATGAAAGAAAGATAAGGGGACAGAACA668000.10105157934315112No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAAGC5329800.079.2938161
AAGCCTT5798500.073.496464
AGCCTTC5832850.073.2148365
CCTTCTA5930850.071.958387
CTTCTAT6043500.070.616078
GCCTTCT6141250.069.5985956
AGCCTAA498300.060.1337975
TTCTATT7232250.058.987569
CACAGCC510550.058.707282
CCACAGC558500.057.1377941
CAGCCTA524600.057.0881424
ACAGCCT536200.055.9786383
CCTAATC561200.053.469317
CTAATCC619850.048.2960668
GCCTAAT626200.048.002296
CGAGTAG245900.046.8365943
TAATCCT668900.044.6978879
CAAAGCG204550.043.2315144
GTAGACC278950.041.9455226
AAGCGAG211550.041.5718426
Produced by FastQC (version 0.11.2)