FastQCFastQC Report
Tue 20 Dec 2016
C9LUFACXX_l02n01_reseq-total_drought_1h_rep3_shoot.35100000073acf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC9LUFACXX_l02n01_reseq-total_drought_1h_rep3_shoot.35100000073acf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121959519
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTT53959214.424354117041082No Hit
TTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAG21990241.8030769701543345No Hit
TACCATGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTC19267031.5797889461994352No Hit
GACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCT14532781.1916068642415685No Hit
CTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCC9154890.7506498939209493No Hit
GCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTCTGCAA7742050.6348048978448332No Hit
TGACTGCAATTTTAGAGAGACGCGAAAGTACAAGCCTGTGGGGTCGCTTC6191380.5076586108871092No Hit
GCCCCTCCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTT4226000.3465084180924No Hit
ACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCC4208420.34506695619224276No Hit
GCAACTGGATAACTAGCACCGAAAACCGTCTTTACATCGGATGGTTCGGT4145760.3399291858473138No Hit
CATGCATTGGATGGATGCCCGGGCATTGAGAAGGAAGGACGCTTTCAGAG4075630.33417891718644777No Hit
AGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATAAACCAAGATCT3932680.3224578148754424No Hit
ACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTG3917790.3212369179645584No Hit
AGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGTCT3600180.2951946702905576No Hit
ACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCA3430000.2812408599282849No Hit
CCAGTAGATATTGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTA3008360.24666873276205692No Hit
CCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTG2910350.2386324596770507No Hit
ATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAATTTGAATA2721400.22313961405505378No Hit
ACAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCG2569860.21071417967793069No Hit
TGCACTTTTACCCAATTTGGGAAGCTGCATCTGTTGATGAGTGGTTATAC2511130.2058986474028321No Hit
GATACCGTCCTAGTCTCAACCATAAACGATGCCGACCAGGGATCGGCGGA2203280.18065666526611998No Hit
GGTACTTTCAACTTTATGATTGTATTCCAGGCAGAGCATAACATCCTTAT1975610.1619889957093058No Hit
CAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT1949170.15982106324968368No Hit
CAACACGGGGAAACTTACCAGGTCCAGACATAGCAAGGATTGACAGACTG1900550.15583449455880521No Hit
GATCCCTACCTTATTGACCGCAACTTCTGTATTTATTATCGCCTTCATCG1885500.1546004785407525No Hit
TGATGGTATTCGTGAGCCTGTTTCTGGTTCTTTACTTTACGGAAACAATA1753270.14375835641004783No Hit
AGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCAA1723000.14127638532257578No Hit
CCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTAGAT1703480.13967585424799847No Hit
CTACGCATTTCACCGCTCCACCGGAAATTCCCTCTGCCCCTACCGTACTC1691120.138662403219219No Hit
CGTACTAGGTTGAATTACTATCGCGACGCGGGCATCAGTAGGGTAAAACT1665170.1365346480253009No Hit
CCACAGCCTAATCCTTATTTTATTCCTACAAATAGAACATAGCCATACGA1639290.13441263244076915No Hit
CACAAGGATGAAATATCAGTAATTTTCTATTTACTGGTTGATCCCATCTT1613940.13233407389873356No Hit
GTAGTTGATAGCCAAGGTCGCGTTATTAATACTTGGGCTGATATCATCAA1586770.1301062855126544No Hit
TTACATCGGATGGTTCGGTGTTTTGATGATCCCTACCTTATTGACCGCAA1557660.12771942795215516No Hit
AAACGGATTCAATTTCAACCAATCTGTAGTTGATAGCCAAGGTCGCGTTA1556120.12759315654565676No Hit
GGTTCTTTACTTTACGGAAACAATATTATCTCTGGTGCCATTATTCCTAC1488780.1220716523160443No Hit
AAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCTTGCA1479960.12134846153337157No Hit
ACCACCAGAGCACAAGAAAGCATAAGCAACACAAGGGATTAACTCATCAC1443110.1183269671635881No Hit
TGACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTA1394520.11434285830530375No Hit
GTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTAGATATTGATGGTAT1347900.11052027845403359No Hit
ACCGCAACTTCTGTATTTATTATCGCCTTCATCGCTGCCCCTCCAGTAGA1323930.10855487221132776No Hit
CAAGCCTGTGGGGTCGCTTCTGCAACTGGATAACTAGCACCGAAAACCGT1310570.10745942676274411No Hit
TGCTCGTGAAGGTAATGAAATTATCCGATCAGCTTGCAAATGGAGTCCTG1309260.10735201407280066No Hit
TAACAAGCCTTCTATTATCTTTCTAGTTAATACGTGTGCTTGGGAGTCCT1267560.10393284676696699No Hit
GGGTATGCGTCCTTGGATTGCTGTTGCATATTCAGCTCCTGTTGCAGCTG1256430.10302024887454664No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACAAGC7089450.077.173311
AGCCTTC7500700.073.6825
AAGCCTT7498600.073.570014
CCTTCTA7685750.071.927527
CTTCTAT7887000.070.1030048
GCCTTCT8116150.068.221246
TTCTATT9524950.058.040359
CCTAGTC446150.050.782299
TACCGTC464900.048.933173
CCTGGTT811850.045.8462
TACGCAT413600.044.7598532
ATACCGT525300.043.2977032
GATACCG528200.043.2019581
CTACGCA446750.042.4767271
CAACACG623750.041.7091181
GTCCTAG552150.041.2733577
ACCGTCC554800.041.122874
TCCTAGT564450.040.27378
AACACGG678300.039.0136572
CGTCCTA589150.038.882356
Produced by FastQC (version 0.11.2)